Select type of query desired Updated May 2020 Home | Kinexus | Contact | Credits
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About KINECTOR...

KiNECTOR is an open-access, knowledgebase developed by Kinexus Bioinformatics Corporation to foster the linkage of proteins in kinase-based cell signalling pathways. Over 21,450 human kinase-substrate relationships (KSRs) were retrieved from several sources, including the PhosphoNET, PhosphoSite Plus, and PhosphoNetworks websites and the scientific literature. This is presented in a graphic format. Functional information is provided for nearly 6000 of these KSRs, and KiNECTOR serves as a portal to other reputable websites that contain detailed information on these kinases and other phosphoproteins. KiNECTOR also provides direct links to antibody products available from Kinexus that faciltate further studies of these regulatory proteins. Over 80% of the 21,300 proteins encoded by the human genome appear to be subject to phosphorylation. While at least 250,000 and possibly a million phosphosites exist in the human phosphoproteome, only a small fraction of these are likely to be consequential. Future studies will need to define the most important KSRs for a comprehensive understanding of the regulatory networks that govern cellular communications and control.

Instructions...

KiNECTOR is designed to be intuitive and simple to navigate. You can use the UniProt ID or common short names for the kinases and substrate proteins of interest in order to find direct KSRs. Lists of possible options for proteins are generated by typing at least two letters of their names or identification numbers, and waiting for a few moments for a complete list of options to appear. Follow the steps outlined on the query webpages when searching for connections. Three possible types of queries are available. The Kinase- and Substrate-based searches only visualize direct connections. For some queries, the number of KSRs may be too large and clear visual representation is not possible on a single map. By activating the available filters, simpler maps can be generated. Extra map pages can be shown by clicking "Previous" or "Next" buttons. Hovering the cursor above the phosphosite on an arrow (edge) generates a pop-up that provides function information and links to websites with further information on the phosphosite. Hovering the cursor above a kinase or substrate icon (node) provides links to websites with more information about the protein. The third type of query permits the user to find direct and indirect connections between a starting protein kinase of interest and a phosphorylated target protein at one phosphorylation site that can be specified or multiple sites. Clicking on a protein icon will generate a new map of direct kinase-substrate interactions with this protein as the central hub.

Other Useful Online Resources From Kinexus...

KiNECTOR is one of several useful open-access, online resources that are available from Kinexus to promote cell signalling research by academic and industrial scientists. Direct links to several of our other knowledgebases in the SigNET Knowledge-Bank are provided just below the header at the top of each webpage in KiNECTOR. TranscriptoNET provides gene expression data on over 21,000 human genes in over 600 diverse human cell lines, organs and tissues. KinaseNET offers detailed information about 536 human protein kinases, whereas OncoNET provides expression and mutation data for 3000 cancer-related proteins in diverse human tissues and cell lines. PhosphoNET features data on nearly 1 million confirmed or predicted phosphorylation sites in over 21,000 human proteins, including known and predicted kinases and phosphatases that target these phosphosites, and the evolutionary conservation of these phosphosites in over 20 other species. KinATLAS reveals interactions between these proteins with each other in signalling systems as well as interactions with kinase inhibitory compounds. Our DrugKiNET knowledgebase features comprehensive information on over 800 compounds that have been experimentally determined to inhibit human protein kinases. This includes the retrieval of the lowest reported compound IC50, Ki and Kd values from several reputable sources for over 105,000 experimentally tested, non-redundant kinase-compound pairs. Using this information for training, we developed two kinase inhibitor prediction algorithms to further predict another 200,000 possible kinase-compound interactions. DrugProNET provides identification of the critical amino acids in drug targets for binding of over 800 drugs. For semi-quantitative data on actual protein expression in over 2,000 diverse biological specimens, please also visit our open-access KiNET-AM DataBank with over 2000 antibody microarray analyses with over 1700 diverse pan- and phosphosite-specific antibodies. Most of these antibodies are available from Kinexus for screening lysates from cells and tissues using our KinexTM KAM antibody microarrays or as stand-alone products at our www.kinexusproducts.ca website.


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